Human interferon‐γ lacking 23 COOH‐terminal amino acids is biologically active

FEBS Letters

Nishimura, Osamu²  Honda, Susumu¹  Sakaguchi, Masao²  Ozawa, Mariko² 

[1] Applied Microbiology Laboratories, Central Research Division, Takeda Chemicals Industries, 2-17-85, Jusohonmachi, Yodogawa-ku, Osaka 532, Japan;Biotechnology Laboratories, Central Research Division, Takeda Chemicals Industries, 2-17-85, Jusohonmachi, Yodogawa-ku, Osaka 532, Japan

关键词: IFN-γ;    Antiviral activity;    Higher structure;   

DOI  :  10.1016/0014-5793(88)80671-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We constructed five mutated cDNAs encoding human interferon-γ (IFN-γ) derivatives lacking 19–23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and behaved as a dimer. The far- and near-UV circular dichroism spectra of the derivatives were quite similar to those of the intact one. These results indicate that the 23 COOH-terminal amino acids at least are not essential for achieving the full antiviral activity and constructing the higher structure of human IFN-γ.

【 授权许可】

Unknown