Anti‐HIV‐1 property of trichosanthin correlates with its ribosome inactivating activity

FEBS Letters

Wang, Jian-Hua¹  Tam, Siu-Cheung²  Huang, Hai²  Nie, Hui-Ling³  Zheng, Yong-Tang¹ 

[1] Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China;Department of Physiology, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, PR China;Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, PR China

关键词: Trichosanthin;    Ribosome inactivating protein;    human immunodeficiency virus type 1;    Antiviral activity;   

DOI  :  10.1016/S0014-5793(02)03539-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti-tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti-HIV-1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site-directed mutagenesis. The anti-HIV-1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCS_M(120–123), TCS_E160A/E189A, with the greatest decrease in RI activity, lost almost all of the anti-HIV activity and cytopathic effect. Another mutant TCS_R122G, which exhibited a 160-fold decrease in RI activity, retained some anti-HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti-HIV-1 property.

【 授权许可】

Unknown