tRNA specificity of a mischarging aminoacyl‐tRNA synthetase: Glutamyl‐tRNA synthetase from barley chloroplasts

FEBS Letters

Schön, Astrid¹  Söll, Dieter¹ 

[1] Department of Molecular Biophysics and Biochemistry, Yale University, PO Box 6666, New Haven, CT 06511, USA

关键词: tRNA;    Glutamyl-tRNA synthetase;    Misaminoacylation;    Substrate specificity;    Chloroplast;   

DOI  :  10.1016/0014-5793(88)80007-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutamyl-tRNA synthetase (GluRS) from barley chloroplasts is a naturally occurring mischarging enzyme: it efficiently aminoacylates both the homologous tRNA^Glu as well as tRNA^Gln species. In order to shed light on the structural requirements of this enzyme for tRNA specificity heterologous charging experiments were performed. Sequence comparison of tRNAs recognized by barley chloroplast GluRS reveals that two base pairs, one in the acceptor stem and one in the anticodon stem, appear to be critical for substrate recognition.

【 授权许可】

Unknown