In vivo formation of glutamyl‐tRNAGln in Escherichia coli by heterologous glutamyl‐tRNA synthetases

FEBS Letters

Orellana, Omar¹  Min, Bokkee²  Söll, Dieter²  Lefimil, Claudia¹  Núñez, Harold¹ 

[1] Programa de Biologı́a Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 838-0453, Chile;Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA

关键词: Misacylated tRNA;    tRNA specificity;    Glutamyl-tRNA synthetase;    Glutaminyl-tRNA synthetase;   

DOI  :  10.1016/S0014-5793(03)01460-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two types of glutamyl-tRNA synthetase exist: the discriminating enzyme (D-GluRS) forms only Glu-tRNA^Glu, while the non-discriminating one (ND-GluRS) also synthesizes Glu-tRNA^Gln, a required intermediate in protein synthesis in many organisms (but not in Escherichia coli). Testing the capacity to complement a thermosensitive E. coli gltX mutant and to suppress an E. coli trpA49 missense mutant we examined the properties of heterologous gltX genes. We demonstrate that while Acidithiobacillus ferrooxidans GluRS1 and Bacillus subtilis Q373R GluRS form Glu-tRNA^Glu, A. ferrooxidans and Helicobacter pylori GluRS2 form Glu-tRNA^Gln in E. coli in vivo.

【 授权许可】

Unknown