Co‐transcription of Rhizobium meliloti lysyl‐tRNA synthetase and glutamyl‐tRNA synthetase genes

FEBS Letters

Pelchat, Martin²  Laberge, Serge¹  Gagnon, Yves²  Lapointe, Jacques² 

[1] Station de recherches sur les sols et les grandes cultures, Agriculture et Agroalimentaire Canada, Sainte-Foy, Que. G1V 2J6, Canada;Département de Biochimie, Faculté des Sciences et de Génie, Pavillon Charles-Eugène Marchand, room 3210, Université Laval, Sainte-Foy, Que. G1K 7P4, Canada

关键词: Co-transcription;    Rhizobium meliloti gltX;    Rhizobium meliloti lysS;    Glutamyl-tRNA synthetase;    Lysyl-tRNA synthetase;   

DOI  :  10.1016/S0014-5793(99)00385-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypeptide of the expected mass (62 kDa), thus confirming that it encodes R. meliloti lysyl-tRNA synthetase. Reverse transcription/polymerase chain reaction was used to demonstrate that this lysS gene is co-transcribed with gltX in R. meliloti. This is the first reported case of two immediately adjacent and co-transcribed genes encoding aminoacyl-tRNA synthetases.

【 授权许可】

Unknown