FEBS Letters | |
Enzyme and organic solvents: Horse liver alcohol dehydrogenase in non‐ionic microemulsion: Stability and activity | |
Lee, Kang Min1  Biellmann, Jean-François1  | |
[1] Laboratoire de Chimie Organique Biologique, UA no. 31, Institut de Chimie, Université Louis Pasteur, 1 rue Blaise Pascal, 67008 Strasbourg, France | |
关键词: Alcohol dehydrogenase; Enzyme stability; Microemulsion; Organic solvent; Surfactant; Water content; | |
DOI : 10.1016/0014-5793(87)80504-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In a microemulsion made with Triton X-100, the stability of the enzymatic activity was higher than in ionic microemulsions. The stability increased with water content. The kinetic constants (Michaelis constant of NAD+ and maximum velocity) were close to those found in the previously studied microemulsions. The Michaelis constant of NAD+ expressed with respect to the buffer volume was higher than in water. The pH dependence of the kinetic constants in this microemulsion was determined. The activity determined by NAD+ reduction decreased with water content, whereas the redox activity determined via butanol oxidation coupled to retinal reduction was only slightly reduced.
【 授权许可】
Unknown
【 预 览 】
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RO201912020289800ZK.pdf | 201KB | download |