Screening of Thermophilic Bacteria Produce Xylanase from Sapan Sungai Aro Hot Spring South Solok

2nd International Conference on Mathematics, Science, Education and Technology

数学;自然科学;教育

Irdawati, I.^1 ; Syamsuardi, S.^2 ; Agustien, A.^2 ; Rilda, Y.^2

Department of Biology, Universitas Negeri Padang, Indonesia^1

Department of Biology, Andalas University, Indonesia^2

关键词: Biochemical characteristics;    Descriptive studies;    Enzyme stability;    High temperature;    Hydrolyzing enzymes;    Industrial sector;    Thermophilic bacteria;    Xylanase productions;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/335/1/012021/pdf
DOI  :  10.1088/1757-899X/335/1/012021

来源: IOP

【 摘 要 】

xylanase is one of the enzymes with great prospects as hemicellulose hydrolyzing enzyme. Global annual market demand for this enzyme reach US $ 200 million. This enzyme catalyzes the xylan (hemicellulose) reactions breaking into xilooligosakarida and xylose. Xylanase can be applied to various industrial sectors such as bread, sugar xylose, biofuels, especially in bleaching paper (bleaching) pulp. Xylanase Isable to replace conventional chemical bleaching using chlorine that is not friendly for the environment. Currently xylanase production is extracted from the thermophilic bacteria for enzyme stability at high temperatures that are suitable for industrial applications. Thermophilic bacteria can be isolated from a hot spring, one of the which is a source of Sapan Sungai Aro Hot Spring, located in the district South Solok. The aim of this study was to select and identification of thermophilic bacteria can produce xylanase.This roomates is a descriptive study, which was Carried out in the Laboratory of Microbiology, Mathematic and Science Faculty of Padang State University, and Laboratory of Bacteriology, BasoVeterinary Research Center. The research procedure consisted of the preparation and sterilization of materials and tools, medium manufacturing, regeneration, selection and identification. Selection is performed by using a semiquantitative screening plate that contains xylan substrate. Identification is based on microscopic and biochemical characteristics until the genus level.Selection results Showed 12 out of 16 isolates had xilanolitik activity, with the highest activity is SSA2 with xilanolitik index of 0.74. The top five index producehigestxilanolitik isolates that are SSA2, SSA3 and SSA4 identified as Bacillus sp. 1., and SSAS6 and SSA7 is Bacillus sp. 2.