Effect of temperature on Candida antartica lipase B activity in the kinetic resolution of acebutolol

International Conference on Chemical and Bioprocess Engineering

地球科学;化学;生物科学

Rajin, Mariani^1 ; Kamaruddin, A.H.^2

Universiti Malaysia Sabah, Faculty of Engineering, Sabah, Kota Kinabalu, Malaysia^1

Universiti Sains Malaysia, School of Chemical Engineering, Nibong Tebal, Penang, Malaysia^2

关键词: Acebutolol;    Candida antartica lipase b;    Effect of temperature;    Enzyme stability;    First order kinetics;    Kinetic resolution;    Lipase deactivation;    Thermodynamic studies;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/36/1/012061/pdf
DOI  :  10.1088/1755-1315/36/1/012061

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Thermodynamic studies of free Candida antartica lipase B in kinetic resolution of acebutolol have been carried out to characterize the temperature effects towards enzyme stability and activity. A decreased in reaction rate was observed in temperature above 40oC. Thermodynamic studies on lipase deactivation exhibited a first-order kinetic pattern. The activation and deactivation energies were 39.63 kJ/mol and 54.90 kJ/mol, respectively. The enthalpy and entropy of the lipase deactivation were found to be 52.12 kJ/mol and -0.18 kJ/mol, respectively.