Methylation of the enhancer region of avian sarcoma virus long terminal repeat suppresses transcription

FEBS Letters

Gowda, Siddareme²  Wagner, Herbert¹  Simon, Dietrich¹  Guntaka, Ramareddy V.² 

[1] Robert Koch Institute, Nordufer 20, Berlin 65, Germany;>Department of Microbiology, School of Medicine, University of Missouri-Columbia, Columbia, MO 65212, USA

关键词: Methylation;    Enhancer;    Long terminal repeat;    Transfection;    Transcription;   

DOI  :  10.1016/0014-5793(87)80951-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of methylation of an enhancer on transcription was studied. A 245 bp enhancer-containing a fragment of the LTR of the avian sarcoma virus was methylated in vitro and ligated back into a vector which lacked the upstream enhancer sequence. The transient expression in QT6 cells indicated that methylation of the enhancer-containing sequence severely reduced the extent of transcription.

【 授权许可】

Unknown