Solitary HERV‐K LTRs possess bi‐directional promoter activity and contain a negative regulatory element in the U5 region

FEBS Letters

Leib-Mosch, Christine²  Domansky, Andrey N.¹  Snezhkov, Eugene V.¹  Kopantzev, Eugene P.¹  Sverdlov, Eugene D.¹  Lebedev, Yuri B.¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117871, Russia;Institut für Molekulare Virologie, GSF-Forschungzentrum fur Umwelt und Gesundheit, Ingolstadter Landstrasse 1, D-85758 Oberschleisheim, Germany

关键词: Human endogenous retrovirus;    HERV-K;    Long terminal repeat;    Regulation of transcription;   

DOI  :  10.1016/S0014-5793(00)01460-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reporter gene analysis of HERV-K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene. Deletion analysis demonstrated the possible existence of alternative promoters within the LTR as well as a silencer-like element in the U5 region. Our results indicate also that all-trans-retinoic acid is capable of modulating expression of the reporter gene directed by a HERV-K LTR in NT2/D1 cells.

【 授权许可】

Unknown