12‐O‐Tetradecanoylphorbol‐13‐acetate induces the enhancer function of human T‐cell leukemia virus type I

FEBS Letters

Ohtani, Kiyoshi²  Nakamura, Masataka²  Sugamura, Kazuo¹  Hinuma, Yorio²  Fujii, Masahiro² 

[1] Department of Bacteriology, Tohoku University School of Medicine, Sendai 980, Japan;Institute for Virus Research, Kyoto University, Kyoto 606 Japan

关键词: Enhancer;    Gene expression;    Long terminal repeat;    Retrovirus;    Tumor promoter;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    4α-PDD;    α-phorbol-12;    13-didecanoate;    HTLV-I;    human T-cell leukemia virus type I;    LTR;    long terminal repeat;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/0014-5793(87)80308-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phorbol esters were employed in studies on the molecular mechanism of the induction of expression of human T-cell leukemia virus type I (HTLV-I) by a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA). Experiments using the chloramphenicol acetyltransferase (CAT) system showed that CAT expression directed by the long terminal repeat (LTR) of HTLV-I was induced by TPA, but not by 4α-phorbol-12,13-didecanoate, which is not an activator of protein kinase C, and that like other known enhancers, irrespective of its position and orientation, a 230-bp fragment in the U3 region of the HTLV-I LTR confers susceptibility to induction by TPA.

【 授权许可】

Unknown