A simple method for extraction of DNA from fungi and yeasts with anhydrous hydrogen fluoride

FEBS Letters

Gunge, Norio¹  Oshiro, Satoru²  Kitada, Kunio¹  Katsura, Nobuhiko² 

[1] Department of Applied Microbial Technology, Kumamoto Institute of Technology, 4-22-1, Ikeda, Kumamoto 860, Japan;Department of Oral Biochemistry, School of Dentistry, Nagasaki University, 7-1, Sakamoto-machi, Nagasaki 852, Japan

关键词: Hydrogen fluoride;    DNA;    Cell wall;    (Saccharomyces cerevisiae;    Fungi;    Yeast);    YPG;    0.5% yeast extract;    1% polypeptone and 2% glucose;    pH 6.0;    TE;    10 mM Tris;    1 mM EDTA;    pH 8.0;    HF;    anhydrous hydrogen fluoride;   

DOI  :  10.1016/0014-5793(87)80851-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel method is described for the extraction of DNAs from fungi and yeasts. Anhydrous hydrogen fluoride (HF) selectively cleaves their cell walls under mild conditions (for 5 min at 0°C), enabling the effective extraction of DNAs from organisms with a cell wall. A possible mechanism for this method concerning the selective cleavage of O-glycosidic linkages in cell walls has been described previously [(1977) Anal. Biochem. 82, 289–309]. The extracted DNA is intact: in fact, the yeast DNA is directly applicable for restriction analysis and transformation of Escherichia coli.

【 授权许可】

Unknown