Enzymatic synthesis of nucleoside 5′‐mono and ‐triphosphates

FEBS Letters

Zinchenko, Anatoli I.¹  Pricota, Tamara I.¹  Sivets, Grigorii G.¹  Poopeiko, Nikolai E.¹  Barai, Vladimir N.²  Zaiashko, Larisa M.²  Mikhailopulo, Igor A.¹ 

[1] Institute of Bio-Organic Chemistry. Byelorussian SSR Academy of Sciences, Zhodinskaya 5/2, Minsk 220600, USSR;Institute of Microbiology, Byelorussian SSR Academy of Sciences, Zhodinskaya 2, Minsk 220733, USSR

关键词: Nucleoside 5'-monophosphate;    Nucleoside phosphotransferase;    Nucleoside 5'-triphosphate;    Nucleotide kinase;    (Saccharomyces cerevisiae;    Erwinia herbicola);    NTP(3'F);    3'-fluoro-3'-deoxyribunocleoside 5'-tri-phosphates with the bases adenine (ATP(3'F)];    guanine [GTP(3'F)];    uracil [UTP(3'F)] and cytosine [CTP(3'F)];    NMP(3'F);    3'-fluoro-3'-deoxyribonucleoside 5'-monophosphates with the natural bases;    Ado(3'F);    Guo(3'F);    Urd(3'F);    and Cyt(3'F);    3'-fluoro-3'-deoxyribonucleosides;    dNTP(3'F) and dNMP(3'F);    3'-fluoro-2';    3'-di-deoxyribonucleoside 5'-tri- and -monophosphates;    respectively;    dAdo (3'F);    dGuo(3'F);    dThd(3'F) and dCyd(3'F);    3'-fluoro-2';    3'-dideoxyribonueleosides;    PNP;    p-nitrophenylphosphate;    NMP and NTP;    nucleoside 5'-mono- and -triphosphates;    respectively;   

DOI  :  10.1016/0014-5793(90)80116-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transformation of fluorodeoxy-nucleosides into 5'-monophosphates with the use of whole bacterial cells of Erwinia herbicola as a biocatalyst and PNP as a phosphate donor and next into 5'-triphosphates by using an extract of the Saccharomyces cerevisiae cells has been demonstrated.

【 授权许可】

Unknown