Transacylase activity of lactating bovine mammary fatty acid synthase

FEBS Letters

Kumar, Soma¹  Anderson, Gregory J.¹ 

[1] Department of Chemistry, Georgetown University, Washington, DC 20057, USA

关键词: Transacylase;    Fatty acid synthase;    Acyl exchange;    Substrate specificity;   

DOI  :  10.1016/0014-5793(87)80839-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An assay for the transacylation reaction catalyzed by fatty acid synthase was developed which does not require model substrates or labelled acyl-derivatives of CoA. It involves the transfer of the acyl group from unlabelled CoA to [³H]CoA. This assay shows the occurrence of transacylation at a relatively high rate with a variety of substrates that the enzyme is able to utilize. The activity is unaffected by dissociation of the enzyme or modification by iodoacetamide or 2-chloroacetyl-CoA.

【 授权许可】

Unknown