[3H]Tetrahydrocerulenin, a specific reagent for radio‐labelling fatty acid synthases and related enzymes

FEBS Letters

Roberts, Graham¹  Leadlay, Peter F.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, England

关键词: Fatty acid synthase;    Cerulenin;    Affinity labelling;    Macrolide antibiotic synthase;    Erythromycin;   

DOI  :  10.1016/0014-5793(83)80407-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have synthesised [³H]tetrahydrocerulenin, a radio-labelled derivative of cerulenin, an antibiotic from Cephalosporium caerulens which specifically inhibits the condensing enzyme of fatty acid synthases and polyketide synthases from various sources. [³H]Tetrahydrocerulenin binds to fatty acid synthase from pig liver with the same specificity as cerulenin. We have also used [³H]tetrahydrocerulenin to monitor condensing enzyme activity in cell-free extracts of the erythromycin-producing organism Streptomyces erythreus.

【 授权许可】

Unknown