Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3‐oxoacyl‐ACP reductase

FEBS Letters

O'Hara, Paul¹  Slabas, Antoni R¹  Fawcett, Tony¹ 

[1] Department of Biological Sciences, University of Durham, Durham DH1 3LE, UK

关键词: 3-Oxoacyl-ACP reductase;    Fatty acid synthase;    [3H]NADPH;    Brassica napus;   

DOI  :  10.1016/S0014-5793(00)02406-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.

【 授权许可】

Unknown