Primary structure of glucagon from the gut of the common dogfish (Scyliorhinus canicula)

FEBS Letters

Thim, Lars³  Conlon, J.Michael¹  O'Toole, Liam² 

[1] Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft, University of Göttingen, Gosslerstrasse 10d, D-3400 Göttingen, FRG;Department of Zoology, University of Sheffield, Sheffield, England;Novo Research Institute, Bagsvaerd, Denmark

关键词: Edman degradation;    HPLC;    Radioimmunoassay;    Posttranslational processing;    (Elasmobranch);   

DOI  :  10.1016/0014-5793(87)80011-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The primary structure of glucagon isolated from the intestine of the common dogfish, Scyliorhinus canicula, was established as 5 10 15 20 25 H S E G T F T S D Y S K Y M D N R R A K D F V Q W L M N T. The peptide shows four substitutions compared with human glucagon: Glu-3 for Gln, Met-14 for Leu, Asn-16 for Ser and Lys-20 for Gln. Glucagon represented the predominant molecular form of the glucagon-like immunoreactivity in the dogfish gut extracts demonstrating that the pathway of posttranslational processing of proglucagon in the gut of this fish differs markedly from the pathway in the mammalian gut.

【 授权许可】

Unknown