Regulation of protein synthesis in rabbit reticulocyte lysates

FEBS Letters

Ranu, Rajinder S.¹  Roberts, Richard C.¹ 

[1] Department of Microbiology, Colorado State University. Fort Collins, CO 80523, USA

关键词: Protein kinase;    Translational inhibitor;    Initiation factor eIF-2;    Phosphorylation;    Ternary complex Heme regulation;   

DOI  :  10.1016/0014-5793(86)81103-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The specificity of the heme-regulated protein kinase (HRI) was investigated further by utilizing the isolated 38000 Da subunit (α subunit) polypeptide of eIF-2 as the substrate. For this purpose, the three subunit polypeptides of eIF-2 (38000 Da, α, 50000 Da, β; and 52000 Da, γ) were resolved by reversed-phase high performance liquid chromatography (HPLC). Results show that HRI is incapable of phosphorylating the 38000 Da subunit separated from the other two eIF-2 polypeptides. Data suggest that the substrate'specificity of HRI is determined by the quaternary structure assumed by the α subunit in association with the other two subunits in the eIF-2 holoprotein.

【 授权许可】

Unknown