The formation of inositol phosphate derivatives by isolated membranes from Acer pseudoplatanus is stimulated by guanine nucleotides

FEBS Letters

Berta, P.²  Dillenschneider, M.¹  Ranjeva, R.¹  Graziana, A.¹  Haiech, J.²  Alibert, G.¹  Hetherington, A.¹ 

[1] Centre de Physiologie Végétale, Université P. Sabatier, UA CNRS no.241, 118, Route de Narbonne, F-31062 Toulouse cedex, France;Centre de recherche de Biochimie Macromoléculaire, UP CNRS no.8402, Route de Mende, F-34033 Montpellier cedex, France

关键词: Phosphoinositide Phosphoinositol Second messenger G-protein Signal transduction;    PI;    phosphoinositide;    IP;    inositol phosphate;    Gpp[NH]p;    guanosine 5'-[β;    γ-imido]triphosphate;    GTP-γS;    guanosine 5'-[γ-thio]triphosphate;    App[NH]p;    adenosine 5'-[β;    γ-imido]triphosphate;    GDPβS;    guanosine 5'-[β-thio]diphosphate;    IP3;    inositol triphosphate;    DAG;    diacylglycerol;   

DOI  :  10.1016/0014-5793(86)81059-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membranes isolated from Acer pseudoplatanus cell suspension cultures that were prelabelled with [³H]inositol are able to release different water-soluble phosphoinositol derivatives in a time-dependent manner. Addition of adenine, cytidine or uridine nucleotide has no effects on the process. In contrast, guanine nucleotide triphosphates were stimulatory in a dose-dependent manner. The non-hydrolyzable derivative guanosine 5'-[γ-thio]triphosphate was the most effective activator while guanosine 5'[β-thio]diphosphate inhibited the action of GTP. It is suggested that GTP-binding proteins exist in the Acer membrane and play a regulatory role in the formation of potentially important second messengers.

【 授权许可】

Unknown