Histamine and bradykinin stimulate the phosphoinositide turnover in human umbilical vein endothelial cells via different G‐proteins

FEBS Letters

Tkachuk, V.A.¹  Voyno-Yasenetskaya, T.A.¹  Panchenko, M.P.¹  Rybin, V.O.¹  Nupenko, E.V.¹ 

[1] Institute of Experimental Cardiology, 121552 Moscow, USSR

关键词: Protein;    G-;    Phosphoinositide turnover;    (Human umbilical vein endothelial cell);    G-protein;    guanine nucleotide binding protein;    Gi;    inhibitory G-protein;    IP3;    inositol trisphosphate;    IP;    inositol monophosphate;    PI;    phosphoinositide;    GTPγS;    guanosine 5'-O-(3-thiotriphosphate);    GDPßS;    guanosine 5'-O-(3-thiodiphosphate);    PTx;    pertussis toxin;    BoTx;    botulinum toxin;    HUVEC;    human umbilical vein endothelial cells;   

DOI  :  10.1016/0014-5793(89)81496-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The G-proteins which regulate hormonal turnover of phosphoinositide (PI) in human umbilical vein endothelial cells have been investigated. A 40–41 kDa doublet present in the membranes of these cells was selectively ADP ribosylated by pertussis toxin (PTx), and this doublet was G_α2 and G_α3 according to immunoblotting with specific antisera. By contrast, a doublet of 24–26 kDa proteins in the same membrane preparations was ADP ribosylated by the C₃ component of botulinum toxin (BoTx). PTx-dependent ADP ribosylation blocked stimulation of PI turnover by histamine, but did not affect stimulation by bradykinin, whereas BoTx (C₂ + C₃ components) had the opposite effect. Thus two different groups of G-proteins may be involved in hormone-dependent stimulation of PI turnover in human umbilical vein endothelial cells.

【 授权许可】

Unknown