Flow‐force relationships in mitochondrial oxidative phosphorylation

FEBS Letters

Woelders, Henri¹  Putters, Jacques¹  van Dam, Karel¹ 

[1] Laboratory of Biochemistry, University of Amsterdam, PO Box 20151, 1000 HD Amsterdam, The Netherlands

关键词: Mitochondria;    Energy transduction;    Localized chemiosmotic coupling;    Protonmotive force;    Oxidation;    Phosphorylation;    TPMP;    triphenylmethylphosphonium;    DNP;    2;    4-dinitrophenol;    FCCP;    carbonyl cyanide P-trifluoromethoxyphenylhydrazone;    AP5A;    P 1 P 5-di-(adenosine-51-) pentaphosphate;    DMO;    5;    5-dimethyloxazolidine-2;    4-dione;   

DOI  :  10.1016/0014-5793(86)81379-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rates of oxidation and phosphorylation in isolated rat-liver mitochondria have a steep dependence on the protonmotive force (Δ≈sm _h ) across the membrane. These experimentally observed relationships proved to be independent of the way in which δ≈gm _h was varied. These results were obtained when the membrane potential (ΔΨ) was calculated from the distribution of K⁺ (in the presence of valinomycin). When triphenyl-methylphosphonium (TPMP⁺) was used as a probe for ΔΨ, slightly different flow-force relationships were obtained. We conclude that unique relationships exist between Δ≈sm _h + and the rates of oxidation and phosphorylation, and that under some conditions the behaviour of the probe TPMP⁺ is anomalous.

【 授权许可】

Unknown