FEBS Letters | |
Flow‐force relationships in mitochondrial oxidative phosphorylation | |
Woelders, Henri1  Putters, Jacques1  van Dam, Karel1  | |
[1] Laboratory of Biochemistry, University of Amsterdam, PO Box 20151, 1000 HD Amsterdam, The Netherlands | |
关键词: Mitochondria; Energy transduction; Localized chemiosmotic coupling; Protonmotive force; Oxidation; Phosphorylation; TPMP; triphenylmethylphosphonium; DNP; 2; 4-dinitrophenol; FCCP; carbonyl cyanide P-trifluoromethoxyphenylhydrazone; AP5A; P 1 P 5-di-(adenosine-51-) pentaphosphate; DMO; 5; 5-dimethyloxazolidine-2; 4-dione; | |
DOI : 10.1016/0014-5793(86)81379-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The rates of oxidation and phosphorylation in isolated rat-liver mitochondria have a steep dependence on the protonmotive force (Δ≈sm h ) across the membrane. These experimentally observed relationships proved to be independent of the way in which δ≈gm h was varied. These results were obtained when the membrane potential (ΔΨ) was calculated from the distribution of K+ (in the presence of valinomycin). When triphenyl-methylphosphonium (TPMP+) was used as a probe for ΔΨ, slightly different flow-force relationships were obtained. We conclude that unique relationships exist between Δ≈sm h + and the rates of oxidation and phosphorylation, and that under some conditions the behaviour of the probe TPMP+ is anomalous.
【 授权许可】
Unknown
【 预 览 】
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RO201912020288258ZK.pdf | 469KB | download |