The apparent non‐linearity of the relationship between the rate of respiration and the protonmotive force of mitochondria can be explained by heterogeneity of mitochondrial preparations

FEBS Letters

Duszyński, Jerzy¹  Wojtczak, Lech¹ 

[1] Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Pasteura 3, 02-093 Warsaw, Poland

关键词: Protonmotive force;    Respiration;    Resting state;    Proton leak;    Mitochondria;   

DOI  :  10.1016/0014-5793(85)80307-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The apparent non-linear relationship between the rate of respiration and the protonmotive force in mitochondria under resting state conditions is an observation which has led to concepts such as (i) non-chute characteristics of the proton leak through the mitochondrial membrane, or (ii) a slippage of proton pumps. We propose that this non-linearity may be a consequence of a heterogeneity of isolated mitochondria concerning the degree of coupling, since a small proportion of totally uncoupled, or loosely coupled, organelles may considerably contribute to the total respiration but not, or only slightly, to the protonmotive force. This hypothesis is supported by a fairly good fitting of computed relationships with those determined experimentally.

【 授权许可】

Unknown