FEBS Letters | |
The phosphate potential maintained by mitochondria in State 4 is proportional to the proton‐motive force | |
Woelders, Henri1  van der Zande, Wim J.1  van Dam, Karel1  Colen, Anne-Marie A.F.1  Wanders, Ronald J.A.1  | |
[1] Laboratory of Biochemistry, BCP Jansen Institute, University of Amsterdam, PO Box 20151, 1000 HD Amsterdam, The Netherlands | |
关键词: Mitochondria; Energy transduction; Localised chemiosmotic coupling; Proton-motive force Phosphate potential ATP synthase stoichiometry; DNP; 2; 4-dinitrophenol; FCCP; carbonyl cyanide p-trifluoromethoxyphenylhydrazone; S13; 5-chloro-3-tert-butyl-2'-chloro-4'-nitrosalicylanilide; AP5A; P1; P5-di(adenosine-5'-)pentaphosphate; ΔG ex p; extramitochondrial phosphate potential; Δμ̃H+; the electrochemical potential difference for protons between two separate spaces; | |
DOI : 10.1016/0014-5793(85)80534-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Evidence is presented for a proportional relationship between the extramitochondrial phosphate potential (ΔG ex p) and the proton-motive force (ΔgmH+ ) across the mitochondrial membrane in rat-liver mitochondria oxidising succinate in State 4, when ΔgmH+ is varied by addition of uncouplers or malonate. This relationship was found when precautions were taken to minimise interference with the determination of ΔG p ex and ΔgmH+ by intramitochondrial nucleotides, adenylate kinase activity, the quenching method, and ΔgmH+ -dependent changes in matrix volume. A non-proportional ΔG p ex/ΔgmH+ relationship was obtained when these precautions were omitted. Our results do not support mosaic protonic coupling, but are not necessarily in conflict with other localised coupling schemes.
【 授权许可】
Unknown
【 预 览 】
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