Peptides of postulated inhibin activity

FEBS Letters

Johansson, Jan²  Khan, Shafiq⁵  Fairwell, Thomas³  Ritzen, Martin⁵  Lerner, Richard¹  Cekan, Sten⁴  Cederlund, Ella²  Diczfalusy, Egon⁴  Kohan, Sergio⁴  Fröysa, Berit⁴  Jörnvall, Hans⁵ 

[1] Research Institute of Scripps Clinic, La Jolla, CA 92037, USA;Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden;Molecular Disease Branch, NHLBI, NIH, Bethesda, MD 20892, U.S.A.;Reproductive Endocrinology Research Unit, Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden;Pediatrie Endocrinology Unit, Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden

关键词: Inhibin;    Follicle-stimulating hormone;    Luteinizing hormone;    Seminal plasma;    Synthetic peptide;    Pituitary cell culture;   

DOI  :  10.1016/0014-5793(86)80488-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 94-residue polypeptide isolated from human seminal plasma and its chemically synthesized C-terminal 28-residue segment were studied in an in vitro inhibin bioassay utilizing rat pituitary cell cultures. Both peptides have previously been claimed to have inhibin activities, and the effects on the secretion and cellular content of gonadotrophins (FSH and LH) were now assessed in the in vitro assay. No inhibition was found. After 72 h of culture, both the cellular content and the spontaneous as well as the LHRH-stimulated release of bioactive or immunoactive FSH and LH remained unaffected. Similarly, no effects were found on the storage and/or release of prolactin, growth hormone, or thyrotropin. We conclude that both the native 94-residue peptide and the synthetic replicate of its C-terminal 28-residue segment, do not influence the pituitary FSH secretion when assessed in this in vitro system.

【 授权许可】

Unknown