Stable binding interactions among subunits of the chloroplast ATP synthase (CF1‐CF0 as examined by solid support (nitrocellulose)‐subunit reconstitution‐immunoblotting

FEBS Letters

Süss, Karl-Heinz¹ 

[1] Zentralinstitutfür Genetik und Kulturpflanzenforschung, Akademie der Wissenschaften der DDR, 4325 Gatersleben, GDR

关键词: Chloroplast;    ATP synthase;    Subunit renaturation;    Solid support-subunit reconstitution-immunoblotting;    Protein interaction;    CF1 and CF0;    enzymatically active and membrane portions of the chloroplast ATP synthase;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(86)80473-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The technique termed solid support-subunit reconstitution-immunoblotting has been applied to examine stable binding interactions among renatured subunits of the chloroplast ATP synthase. Reconstitution of the CF₁ subunits α, β and γ and subunit III of CF₀ with thylakoid membrane polypeptides separated electrophoretically and transferred to nitrocellulose filters followed by immunodetection of hybrid subunit complexes revealed interactions between α-ϵ, α-CF₀-I, α-CF₀-II, α-δ, β-γ, β-CF₀-II β-ϵ, β-CF₀-III, γ-CF₀-II, γ-ϵ, γ-CF₀-III, CF₀-III-ϵ. Thus, the data confirm results on neighbour relationships of ATP synthase subunits obtained by cross-linking and suggest that the method employed is generally applicable to examine protein-protein binding interactions.

【 授权许可】

Unknown