Proton gradient‐induced changes of the interaction between CF0 and CF1 related to activation of the chloroplast ATP synthase

FEBS Letters

Strotmann, Heinrich¹  Volfson, Irina¹  Ponomarenko, Sophia¹ 

[1] Institut für Biochemie der Pflanzen, Heinrich Heine Universität Düsseldorf, Universitätsstr. 1, D-40225 Düsseldorf, Germany

关键词: ATP synthase;    CF0CF1;    Chloroplast;    Activation;    Protein-protein interaction;    Proton gradient;    CF0CF1;    chloroplast ATP synthase;    DCCD;    dicyclohexyl carbodiimide;    DTT;    dithiothreitol;    PAGE;    polyacrylamide gel electrophoresis;    pmf;    proton motive force;    SDS;    sodium dodecylsulfate;    TCA;    trichloroacetic acid;    Tricine;    N-[2-hydroxy-1;    1-bis(hydroxymethyl)ethyl]glycine;   

DOI  :  10.1016/S0014-5793(98)01681-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thylakoid energization by light causes destabilization of CF₀CF₁ so that the peripheral CF₁ sector is more readily detached from the membrane by intermediate concentrations of the chaotropic salt NaSCN. Here we have investigated the correlation between the proton gradient-induced change of CF₀CF₁ interaction and CF₀CF₁ activation. The results indicate a close relationship between the two phenomena. The effect is most probably due to reduction of the electrostatic interaction between the two subcomplexes CF₀ and CF₁ as a consequence of protonations in the interface region.

【 授权许可】

Unknown