Electron microscopic analysis of the transcription of the Bacillus subtilis riboflavin operon inserted into the hybrid plasmid pLP102

FEBS Letters

Alexandrov, A.A.¹  Osina, N.K.¹  Kalambet, Yu.A.¹ 

[1] Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov Sq., 123I82 Moscow, USSR

关键词: Riboflavin operon;    (Bacillus subtilis);    Transcription;    Electron microscopy;   

DOI  :  10.1016/0014-5793(86)80217-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We used electron microscopy and computer analysis to study the Bacillus subtilis riboflavin operon transcription. The size of the riboflavin operon has been shown to be 2.40 ± 0.15 MDa. Transcription maps of the riboflavin operon in vitro and in vivo have been obtained. In the riboflavin operon region four transcripts are sythesized in vivo having the following approximate boundary positions in the map of pLP102: 46–52, 46–72, 62–72 and 55–58 map units. The riboflavin operon is transcribed from three promoters, at 46, 58 and 72 map positions. A scheme of the riboflavin operon transcription in B. subtilis is suggested.

【 授权许可】

Unknown