Expression of the human apolipoprotein AI gene fused to the E. coli gene for β‐galactosidase

FEBS Letters

Palomba, R.¹  Soria, M.¹  Martineau, D.¹  Monaco, L.¹  Lorenzetti, R.¹  Lappi, D.A.¹  Sidoli, A.¹ 

[1] Laboratory of Molecular Biology, Farmitalia Carlo Erba SpA, 24 Viale Bezzi, 20146 Milano, Italy

关键词: (Human);    Apolipoprotein AI;    HDL;    recombinant DNA;    Cloning;    Gene expression;    β-Galactosidase;   

DOI  :  10.1016/0014-5793(86)80114-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human apoAI gene was expressed in E. coli by in-frame fusion to a modified β-galactosidase gene present in plasmid pUR291. The fused β-galactosidase-apoAI gene product was expressed at a high level and was recognized by an anti-human apoAI antiserum. Besides the fused protein, at least one degradation product having an M _r similar to that of β-galactosidase was present in high amounts in bacterial extracts. These results and those of a pulse-chase experiment indicate that degradation took place only in the apoAI moiety of the chimeric protein.

【 授权许可】

Unknown