Expression of human cathepsin B protein in Escherichia coli

FEBS Letters

Fong, Dunne¹  Chan, Marion Man-Ying¹ 

[1] Bureau of Biological Research and Department of Biological Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ 08855-1059, USA

关键词: Gene expression system;    Bacteriophage T7;    Expression vector;    recombinant DNA;    (Human);   

DOI  :  10.1016/0014-5793(88)80920-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA fragment containing the coding sequence for the mature enzyme of human lysosomal proteinase cathepsin B was inserted in the pET plasmid expression vectors, so that it was placed under the control of transcription and translation signals from bacteriophage T₇. Upon induction, cathepsin B antigen was detected by in situ immunoscreening of lysed E. coli and by Western blot analysis of bacterial lysates. To our knowledge this is the first report of abundant synthesis of cloned cathepsin B in any expression system. Subfragments of cathepsin B can also be generated by this technique and will be used to study cathepsin B structure and function.

【 授权许可】

Unknown