Over‐expression of natural and variant human H‐chain ferritins in E. coli

FEBS Letters

Davison, John¹  Prozzi, Deborah²  Crichton, Robert R.² 

[1] Unit of Molecular Biology, ICP, 75 Avenue Hippocrate, 1200 Brussels, Belgium;Unit of Biochemistry, Universitè Catholique de Louvain, Louvain-la-Neuve, 1200 Brussels, Belgium

关键词: recombinant DNA;    H-ferritin;    Expression vector;    Iron metabolism;    Protein engineering (Human;    E. coli);   

DOI  :  10.1016/0014-5793(88)81303-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The natural human H-chain ferritin was expressed in E. coli using a multi-copy expression vector containing the λ p_L promoter. A variant H-ferritin, having an altered N-terminus, was also produced. These proteins are overproduced (> 30% of the soluble protein), correctly assembled into its 24-subunit shell, and able to bind iron. The identity of the products was confirmed using an antibody specific for H-ferritin.

【 授权许可】

Unknown