Locus‐specific detection of HLA‐DQ and ‐DR antigens by antibodies against synthetic N‐terminal octapeptides of the β chain

FEBS Letters

Kofod, Hans¹  Deufel, Thomas¹  Lernmark, Åke¹  Grove, Anne¹ 

[1] Hagedom Research Laboratory, Niels Steensensvej 6, DK-2820 Gentofte, Denmark

关键词: HLA class II antigen;    Flow cytofluorometry;    ELISA;    Immunoblotting bl];   

DOI  :  10.1016/0014-5793(85)81050-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100–1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding was primarily confined to monocytes and a subpopulation of lymphocytes. It is concluded that locus-specific immunological reagents to distinguish between β chains of HLA-DR and -DQ have been prepared by the preparation by the production of antibodies against the N-terminal sequences of each polypeptide

【 授权许可】

Unknown