Membrane lectins on human monocytes

FEBS Letters

Midoux, Patrick¹  Monsigny, Michel¹  Bouchard, Philippe¹  Roche, Annie-Claude¹ 

[1] Laboratoire de Biochimie Cellulaire et Moléculaire des glycoconjugués. Centre de Biophysique Moléculaire, CNRS and Université d'Orléans, 45071 Orléans Cedex 2, France

关键词: Lectin;    Monocyte;    Endocytosis;    Flow cytofluorometry;   

DOI  :  10.1016/0014-5793(85)80080-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Freshly isolated human monocytes, which do not contain cell-surface mannose-specific receptors, bind mannose 6-phosphate and actively endocytose mannose 6-phosphate-bearing neoglycoproteins (6-P-Man-F-BSA). Three days after isolation, human monocytes endocytose very actively 6-P-Man-F-BSA as well as Man-F-BSA, and the endocytosed neoglycoproteins are rapidly degraded. These results were obtained in quantitative flow cytofluorometry by using a panel of fluoresceinylated sugar-substituted serum albumins (neoglycoproteins). Thus, in contrast to mannose receptors which appear only after maturation, mannose 6-phosphate receptors are already present on freshly isolated human monocytes.

【 授权许可】

Unknown