HLA antigen expression in melanocytic lesions: Is acquisition of HLA antigen expression a biomarker of atypical (dysplastic) melanocytes?

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY

卷:66

Article

Campoli, Michael²  Fitzpatrick, James E.²  High, Whitney²  Ferrone, Soldano^1,3,4,5 

[1] Univ Pittsburgh, Inst Canc, Dept Immunol, Pittsburgh, PA 15213 USA

[2] Univ Colorado, Dept Dermatol, Hlth Sci Ctr, Aurora, CO USA

[3] Univ Pittsburgh, Inst Canc, Dept Surg, Pittsburgh, PA 15213 USA

[4] Univ Pittsburgh, Inst Canc, Dept Pathol, Pittsburgh, PA 15213 USA

[5] Univ Pittsburgh, Sch Med, Canc Immunol Program, Pittsburgh, PA 15213 USA

关键词: atypical nevus;    cancer;    classical HLA class I antigen;    dysplastic nevus;    HLA class II antigen;    immune escape;    immune surveillance;    immunotherapy;    melanocyte;    melanoma;    nevus;   

DOI  :  10.1016/j.jaad.2011.04.025

来源: Elsevier

【 摘 要 】

Background: Although criteria are established for the histologic diagnosis of atypical nevi (AN), consensus about the criteria in the diagnosis of and in the definition of AN is limited. Moreover, intraobserver and interobserver differences in the application of these criteria for the diagnosis of AN have been observed. Objective: We sought to determine the usefulness of HLA antigen expression as a biomarker of AN. Methods: The immunoperoxidase reaction was used to mark common nevi and AN with HLA class I heavy chain(-), beta 2microglobulin (beta 2m)-, and HLA class II beta chain-specific monoclonal antibodies. Results: HLA class I heavy chain, beta 2m, and HLA class II beta chain were expressed in 5 (8.6%) of the 58 common nevi and in 46 (similar to 72%) of the 64 atypical melanocytic lesions. Among common lesions, only halo nevi expressed HLA class I heavy chain, b2m, and HLA class II b chain. The level of HLA class I heavy chain beta 2m and of HLA class II beta chain expression correlated with the degree of cytologic atypia and architectural disorder. Limitations: The number of lesions tested and the subjective nature of the analysis of immunohistochemical staining of tissue sections are both limitations. Conclusions: The data presented suggest that HLA antigen expression is an objective biomarker that correlates well with the degree of cytologic atypia in AN and may: (1) be useful to distinguish common nevi from AN, and (2) represent a more objective measure to determine which AN should be excised. (J Am Acad Dermatol 2012;66:911-6.)

【 授权许可】

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