Rat pancreas contains the proglucagon(64–69) fragment and arginine stimulates its release

FEBS Letters

Iguchi, Kazuaki¹  Kadowaki, Makoto¹  Yanaihara, Chizuko¹  Yanaihara, Noboru¹  Matsumoto, Tomoaki¹ 

[1] Laboratory of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka, Shizuoka 422, Japan

关键词: Rat proglucagon;    Glicentin (64–69);    Isolated rat pancreas perfusion;    Radioimmunoassay;    Arginine-stimulated glucagon release;    Peptide synthesis;   

DOI  :  10.1016/0014-5793(85)81265-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat proglucagon(64–69) corresponding to the C-terminal hexapeptide of putative rat glicentin sequence in the precursor was synthesized. A glicentin C-terminal hexapeptide specific radioimmunoassay, using the synthetic hexapeptide as standard, demonstrated the presence in rat pancreas of a peptide identified with the synthetic rat proglucagon(64–69): H-Asn-Arg-Asn-Asn-Ile-Ala-OH. The hexapeptide was released concomitantly with glucagon by arginine stimulation from the isolated perfused rat pancreas. The results indicate that the pancreas co-stores and possibly co-releases the hexapeptide with glucagon as one of the processing products of proglucagon.

【 授权许可】

Unknown