Detection of the tripeptide Tyr‐Gly‐Gly, a putative enkephalin metabolite in brain, using a sensitive radioimmunoassay

FEBS Letters

Gros, Claude¹  Llorens-Cortes, Catherine¹  Schwartz, Jean-Charles¹ 

[1] Unité de Neurobiologie, Centre Paul Broca de l'INSERM, 2 ter rue d'Alésia, 75014 Paris, France

关键词: Enkephalin metabolism;    Tyr-Gly-Gly;    Radioimmunoassay;   

DOI  :  10.1016/0014-5793(85)81049-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A sensitive and specific radioimmunoassay has been developed for YOG. The tripeptide was previously derivatised with p-benzoquinone to prepare the immunogen and the 125 tracer as well as in samples submitted to the RIA. The sensitivity is about 1 nM as compared with 8000 nM for underivatised YGG. Measurable amounts of endogenous YGG immunoreactivity, co-eluting in HPLC with authentic YGG, were detected in mouse striatal extracts.

【 授权许可】

Unknown