Purification and characterisation of chicken brain hypoxanthine‐guaninephosphoribosyltransferase

FEBS Letters

Veres, Gabor¹  Monostori, Eva¹  Rasko, Istvan¹ 

[1] Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701 Szeged, Hungary

关键词: Enzymology HGPRT Chicken Monoclonal antibody;    MP;    inosine 5'-monophosphate;    GMP;    guanine 5'-monophosphate;    PRPP;    5-phosphoribosyl 1-pyrophosphate;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;    HRPO;    horseradish peroxidase;   

DOI  :  10.1016/0014-5793(85)80626-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hypoxanthine-guanine phosphoribosyltransferase enzyme (EC 2.4.2.8) from chicken brain has been purified 10000-fold to homogeneity. The molecular mass of the native enzyme is 85 kDa, with four subunits, each of 26 kDa, and exerts its maximum activity at pH 10.0. The K_m values for hypoxanthine and guanine are 5.2 and 1.8 μM, respectively. The half-life of the enzyme is 30 min at 85°C. Monoclonal antibodies were raised against the native purified enzyme and were used for purification of enzyme to homogeneity. The monoclonal antibody did not bind to the active centre of the enzyme.

【 授权许可】

Unknown