Biochemical characterization of glucosaminylmuramyldipeptide binding sites of murine macrophages

FEBS Letters

Shebzukhov, Yu.V¹  Andronova, T.M.¹  Golovina, T.N.¹  Sumaroka, M.V.¹  Nesmeyanov, V.A.¹  Samokhvalova, L.V.¹ 

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871 Moscow, Russian Federation

关键词: Muramyl peptide receptor;    Affinity;    labeling;    Radioligand analysis;    MP;    muramyl peptide;    MDP;    MurNAc-Ala-d-iGln;    GMDP;    GlcNAc-β1-4-MurNAc-Ala-d-iGln;    GMDP-Lys;    GlcNAc-β1-4-MurNAc-Ala-d-iGln-Lys;    PBS;    phosphate buffered saline;    BSA;    bovine serum albumin;    Hp;    3-(4-hydroxyphenyl)propionyl;    AzS;    4-azidosalicyl;   

DOI  :  10.1016/0014-5793(94)01219-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By using radioligand analysis, murine peritoneal macrophages were shown to express several hundred high-affinity cell surface GMDP-binding sites (K _a 350 pM). Photoaffinity labeling followed by SDS-PAGE enabled us to identify 32–34 and 38 kDa proteins inside these cells that bound GMDP specifically.

【 授权许可】

Unknown