Highly selective affinity labelling of RNA polymerase B (II) from wheat germ

FEBS Letters

Mustaev, A.A.¹  Grachev, M.A.¹  Maximova, T.G.¹  Sieber, H.²  Schäffner, A.R.²  Hartmann, G.R.²  Zaychikov, E.F.¹ 

[1] Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 90, USSR;Institut für Biochemie der Ludwig-Maximilians-Universität, D-8000 München 2, FRG

关键词: RNA polymerase B (II) (Wheat germ) Affinity labeling 4-[N-(β-Hydroxyethyl)-N-methylamino]benzaldehyde Dinucleotide synthesis;   

DOI  :  10.1016/0014-5793(86)81154-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(β-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH₄. Reaction of the modified enzyme with [α-³²P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with M _r 140000 by covalently linked ApU. Labelling was inhibited by 1 α-amanitin.

【 授权许可】

Unknown