FEBS Letters | |
Membrane interactions of mastoparan analogues related to their differential effects on protein kinase C, Na,K‐ATPase and HL60 cells | |
Kuo, J.F.1  Zheng, Bin1  Vogler, William R.1  Kim, Young-Sook1  Raynor, Robert L.1  | |
[1] Departments of Pharmacology and Medicine (Hematology/Oncology), Emory University School of Medicine, Atlanta, GA 30322, USA | |
关键词: Mastoparan and analogues; Protein kinase C: Na; K-ATPase; HL60 cell; MP; mastoparan; P-MP; polistes mastoparan; TPA; 12-O-tetradecanoylphorbol-13-O-acetate; PDBu; phorbol 12; 13-dibutyrate; PS; phosphatidylserine; PKC; protein kinase C; PKM; protein kinase M; ET-18-OCH3; 1-O-octadecyl-2-O-methyl-3-glycero-3-phosphocholine; IC50; concentration causing 50% inhibition; | |
DOI : 10.1016/0014-5793(92)80694-C | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Membrane interactions of tetradecapeptide toxin mastoparan (MP) and analogues (MP-3, MP-X and polistes MP), as indicated by inhibition of various enzymatic and cellular activities, were investigated. MP-3 was found to be the least active in inhibiting protein kinase C (PKC; activated by phosphatidylserine vesicles, synaptosomal membranes or phorbol ester), synaptosomal membrane Na.K-ATPase and proliferation and viability or leukemia HL60 cells. MP-3, however, was as active as others in inhibiting PKC activated by arachidonate monomers and phorbol ester binding. The unique properties or MP-3, the [des-lle-Asn2]- analogue or MP, might be related to its low functional amphiphilicity compared to others and useful in further delineating biological activities associated with or regulated by membranes.
【 授权许可】
Unknown
【 预 览 】
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RO201912020296642ZK.pdf | 531KB | download |