Changes in the kinetics of inositol transport during TPA‐induced differentiation of HL60 cells towards monocytes

FEBS Letters

Brown, G.²  Bunce, C.M.²  Baxter, M.A.¹  Grafton, G.¹  Sheppard, M.C.¹ 

[1] Department of Medicine, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK;Department of Immunology, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

关键词: Inositol transport;    Monocyte differentiation;    HL60 cell;    Phorbol ester;    DMSO;    dimethylsulphoxide;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(91)80980-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When exposed to the phorbol ester TPA, HL60 cells undergo growth arrest and differentiate towards monocytes. During TPA-induced differentiation there was a 2.6-fold increase in the rate of inositol transport (V _max), a 2.1-fold increase in intracellular inositol and a 1.5-fold increase in inositol lipid. An increase in the V _max of inositol transport did not occur when the variant cell line HL60Ast3 was exposed to TPA, which has been shown in this cell line to induce growth arrest but not differentiation. This observation suggests that the change in inositol transport during HL60 monocyte differentiation is specifically associated with the process of cell differentiation as opposed to growth arrest.

【 授权许可】

Unknown