Relative molecular mass determination of a major, highest relative molecular mass extracellular amelogenin of developing bovine enamel

FEBS Letters

Strawich, E.²  Glimcher, M.J.²  Renugopalakrishnan, V.²  Poon, P.M.¹ 

[1] Department of Chemistry and Biochemistry University of California, Los Angeles, CA 90024, USA;Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedic Surgery, Harvard Medical School, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115 USA

关键词: Relative molecular mass Embryonic enamel Amelogenin precursor;    Ser(P);    O-phosphoserine;    Gdn-HCl;    guanidine hydrochloride;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(85)80604-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins of developing bovine enamel were fractionated by molecular sieving and ion-exchange chromatography. The major fraction corresponding to the highest M _r amelogenin of M _r ~26000–30000 was isolated and its M _r determined by SDS-PAGE, molecular sieving on G-100 resin and high performance liquid chromatography and by sedimentation-equilibrium ultracentrifugation, the latter three procedures in guanidine hydrochloride. SDS-PAGE and HPLC molecular sieving, employing commonly used M _r standards, gave M _r values of ~22000–26000. SDS-PAGE and HPLC molecular sieving, using proline-rich CNBr peptides of collagen as standards, and sedimentation-equilibrium ultracentrifugation, gave M _r values of ~15000–18000 and ~17385, respectively. These latter values correspond well with those reported earlier and with the M _r of the major amelogenin computed from recent amino acid sequence data (~19000). It is concluded that the recently described, highest M _r amelogenin of M _r = 26000–30000 is not a new component but is identical to the proline-rich components having relative molecular masses ranging from 15000 to 18000 described much earlier by several groups of workers.

【 授权许可】

Unknown