FEBS Letters | |
The cysteine proteinase inhibitor chicken cystatin is a phosphoprotein | |
Krieglstein, Kerstin2  Laber, Bernd2  Huber, Robert2  Henschen, Agnes2  Bode, Wolfram2  Kos, Janko1  Turk, Vito1  | |
[1] Odsek za biokemijo, Institut J. Stefan, Univerza E. Kardelja, YU-61000 Ljubljana, Yugoslavia;Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG | |
关键词: Cystatin; Proteinase inhibitor; Protein phosphorylation; (Chicken egg white); PSer; O-phosphoserine; | |
DOI : 10.1016/0014-5793(89)80453-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Peptide maps obtained by reversed-phase HPLC of tryptic digests of isoelectric form 1 (pI=6.5) and 2 (pI=5.6) of chicken egg white cystatin revealed that the difference was located only in a single peptide (residues Ser-74—Lys-91). Ser-80 of cystatin 2 was subsequently identified as being modified by phosphorylation. Moreover, alkaline phosphatase treatment of a mixture of native cystatin forms 1 and 2 was shown by ion-exchange chromatography to cause the disappearance of isoelectric form 2 with a concomitant increase in form 1. Thus, the existence of two isoelectric forms of chicken cystatin is due to the phosphorylated form 2 and non-phosphorylated form 1.
【 授权许可】
Unknown
【 预 览 】
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