期刊论文详细信息
FEBS Letters
The cysteine proteinase inhibitor chicken cystatin is a phosphoprotein
Krieglstein, Kerstin2  Laber, Bernd2  Huber, Robert2  Henschen, Agnes2  Bode, Wolfram2  Kos, Janko1  Turk, Vito1 
[1] Odsek za biokemijo, Institut J. Stefan, Univerza E. Kardelja, YU-61000 Ljubljana, Yugoslavia;Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG
关键词: Cystatin;    Proteinase inhibitor;    Protein phosphorylation;    (Chicken egg white);    PSer;    O-phosphoserine;   
DOI  :  10.1016/0014-5793(89)80453-3
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Peptide maps obtained by reversed-phase HPLC of tryptic digests of isoelectric form 1 (pI=6.5) and 2 (pI=5.6) of chicken egg white cystatin revealed that the difference was located only in a single peptide (residues Ser-74—Lys-91). Ser-80 of cystatin 2 was subsequently identified as being modified by phosphorylation. Moreover, alkaline phosphatase treatment of a mixture of native cystatin forms 1 and 2 was shown by ion-exchange chromatography to cause the disappearance of isoelectric form 2 with a concomitant increase in form 1. Thus, the existence of two isoelectric forms of chicken cystatin is due to the phosphorylated form 2 and non-phosphorylated form 1.

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