Biosynthesis of O‐phosphoserine‐containing phosphoproteins by isolated bone cells of mouse calvaria

FEBS Letters

Sakamoto, M.²  Gotoh, Y.²  Glimcher, M.J.¹  Sakamoto, S.² 

[1] Children's Hospital Medical Center, 300 Longwood Avenue, Boston, MA 02115, USA;Department of Orthopaedic Surgery, Laboratory for the Study of Skeletal Disorders and Rehabilitation, Harvard Medical School, Boston, MA 02115, USA

关键词: Bone cell;    Phosphoprotein synthesis;    Phosphoserine;    Mouse calvaria;    PTH;    parathyroid hormone;    MEM;    minimum essential medium (Eagle);    Ser(P);    O-phosphoserine;    Gdn-HCl;    guanidine hydrochloride;   

DOI  :  10.1016/0014-5793(83)80886-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Five groups of isolated bone cells from mouse calvaria were incubated with [³H]serine and the presence and amount of O[³H]phosphoserine used as an indication of phosphoprotein synthesis. Cells in the osteoblastic fraction were the most active in synthesizing phosphoproteins, and unlike the other cell groups, released most of the phosphoproteins into the tissue culture medium. When subjected to molecular sieving and ion-exchange chromatography, the phosphoproteins synthesized by the bone cells of the osteoblastic group behaved like the phosphoproteins extracted from mouse calvaria by EDTA.

【 授权许可】

Unknown