A structure for the porcine TSH receptor

FEBS Letters

Howells, Roger D.¹  Kajita, Yoshihiro¹  Buckland, Paul R.¹  Rickards, Carole R.¹  Smith, Bernard Rees¹ 

[1] Endocrine Immunology Unit, 7th Floor Medicine, University of Wales College of Medicine, Cardiff CF4 4XN, WalesUK

关键词: Thyrotropin;    Receptor;    Thyroid;    Graves'disease;    Affinity labelling;    Crosslinking;   

DOI  :  10.1016/0014-5793(85)80263-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Affinity purified, detergent-solubilised porcine TSH receptors have been crosslinked to a ¹²⁵I-labelled photoactive derivative of TSH and analysed by gel electrophoresis, gel filtration and sucrose density gradient centrifugation. Our studies suggest that the porcine TSH receptor is made up of a hydrophilic A subunit with an M _r about 45000 linked to an amphiphilic B subunit (M _rapprox. 25 000) by a disulphide bridge(s). The A subunit forms the binding site for TSH on the outside of the cell membrane. The B subunit appears to penetrate the membrane and form the site for interaction with adenylate cyclase either in the lipid bilayer or close to the cytoplasmic surface of the membrane.

【 授权许可】

Unknown