Isolation and structure of the bacterial sex pheromone, cAD1, that induces plasmid transfer in Streptococcus faecalis

FEBS Letters

Craig, Ronald A.²  Kitada, Chieko¹  Mori, Masaaki³  Narita, Michihiko³  Clewell, Don B.²  Isogai, Akira³  Sakagami, Youji³  Fujino, Masahiko¹  Suzuki, Akinori³ 

[1] Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka 532, Japan;Departments of Oral Biology and Microbiology/Immunology, Schools of Dentistry and Medicine, and The Dental Research Institute, University of Michigan, Ann Arbor, MI 48109, USA;Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

关键词: Streptococcus faecalis Sex pheromone Conjugative plasmid transfer Amino acid sequence;    CIA;    clumping-inducing agent;    HPLC;    high performance liquid chromatography;    FAB;    fast atom bombardment;    TFA;    trifluoroacetic acid;    HFBA;    heptafluorobutyric acid;   

DOI  :  10.1016/0014-5793(84)81248-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Streptococcus faecalis sex pheromone cAD1, which is involved in the conjugative transfer of the hemolysin plasmid pAD1, has been isolated and its structure determined. Its M _r is 818 and its amino acid sequence is H-Leu-Phe-Ser-Leu-Val-Leu-Ala-Gly-OH. A replicate of the pheromone synthesized by the liquid phase method showed the same biological activity and Chromatographie behavior as the isolated cAD1. Pheromone activity was detectable at a concentration of ~ 5 × 10⁻¹¹ M.

【 授权许可】

Unknown