期刊论文详细信息
FEBS Letters
Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373
White, Bryan A.1  Tanaka, Hideo2  Kitada, Chieko3  Mori, Masaaki2  Clewell, Don B.1  Isogai, Akira2  Sakagami, Youji2  An, Florence Y.1  Fujino, Masahiko3  Suzuki, Akinori2 
[1] Departments of Oral Biology and Microbiology/Immunology, Schools of Dentistry and Medicine, The University of Michigan, Ann Arbor, MI 48109, USA;The Dental Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA;Central Research Division, Takeda Chemical Industries, Ltd, Yodogawa-ku, Osaka 532, Japan
关键词: (Streptococcus faecalis;    staphylococcus aureus);    Sex pheromone;    Conjugative plasmid transfer Amino acid sequence;    CIA;    clumping-inducing agent;    HPLC;    high-performance liquid chromatography;    TFA;    trifluoroacetic acid;    FAB;    fast atom bombardment;    Boc;    t-butoxycarbonyl;    Bzl;    benzyl;   
DOI  :  10.1016/0014-5793(86)81342-4
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10−11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its M r was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

【 授权许可】

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