Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373

FEBS Letters

White, Bryan A.¹  Tanaka, Hideo²  Kitada, Chieko³  Mori, Masaaki²  Clewell, Don B.¹  Isogai, Akira²  Sakagami, Youji²  An, Florence Y.¹  Fujino, Masahiko³  Suzuki, Akinori² 

[1] Departments of Oral Biology and Microbiology/Immunology, Schools of Dentistry and Medicine, The University of Michigan, Ann Arbor, MI 48109, USA;The Dental Research Institute, The University of Michigan, Ann Arbor, MI 48109, USA;Central Research Division, Takeda Chemical Industries, Ltd, Yodogawa-ku, Osaka 532, Japan

关键词: (Streptococcus faecalis;    staphylococcus aureus);    Sex pheromone;    Conjugative plasmid transfer Amino acid sequence;    CIA;    clumping-inducing agent;    HPLC;    high-performance liquid chromatography;    TFA;    trifluoroacetic acid;    FAB;    fast atom bombardment;    Boc;    t-butoxycarbonyl;    Bzl;    benzyl;   

DOI  :  10.1016/0014-5793(86)81342-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10⁻¹¹ M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its M _r was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

【 授权许可】

Unknown