Primary culture, cellular stress and differentiated function

FEBS Letters

Wolffe, Alan P.¹  Tata, Jamshed R.¹ 

[1] National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, England

关键词: Tissue disaggregation;    Cellular phenotyp;    Hormonal responsiveness;    Heat shock protein;    Culture shock;   

DOI  :  10.1016/0014-5793(84)80902-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolation of specialized cell types for the analysis of tissue-specific gene function often results in loss of the differentiated phenotype. Examples of this type of phenotypic change following tissue disaggregation are reviewed together with possible explanations. Close similarities between the effects of cell isolation with those of other cellular stresses such as heat or anoxia point to common biochemical mechanisms being involved. This suggests that the study of freshly isolated cells will contribute significantly to out understanding of the nature of cellular stress and its consequences for the maintenance of phenotype and induction of tissue specific gene expression.

【 授权许可】

Unknown