Proline content and protein patterns in Eucalyptus grandis shoots submitted to high and low temperature shocks

Brazilian Archives of Biology and Technology

Gustavo M. Souza²  Victor J. M. Cardoso¹  Antonio N. Gonçalves¹ 

[1] ,USP Escola Superior de Agricultura 'Luiz de Queiroz' Departamento de Ciências BiológicasPiracicaba SP ,Brazil

关键词: Heat shock protein;    proline;    recovery;    stress;    temperature shock;   

DOI  :  10.1590/S1516-89132004000300004

来源: SciELO

【 摘 要 】

Proline content and protein patterns changes in response to temperature shocks of both acclimated and non acclimated E. grandis shoots cultivated in vitro were investigated. Analysis of soluble proteins through SDS-PAGE and proline were carried out after 12h at 12ºC (cold acclimation) or 33ºC (heat acclimation), and immediately after temperature shocks at 41ºC and 0ºC. Analyses were also performed 24h after the temperature shocks (recovery period). Temperature treatment at 0ºC did not change soluble protein patterns both in acclimatized and non acclimatized plants, whereas cold temperature induced high proline levels, which kept relatively high after recovery period. Three novel, probably HSPs, proteins (90.5, 75 and 39 kDa) were observed in both acclimated and non acclimated plants submitted to high temperatures. Plants exposed at 41ºC were able to recover from heat shocks after 24h, whereas they did not recover completely from cold shocks. The effect of the acclimation period on the recovering (homeostasis) varied depending on the parameter evaluated, and type and duration of the temperature shock.

【 授权许可】

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