Proteolytic activation of tissue plasminogen activator by plasma and tissue enzymes

FEBS Letters

Ichinose, Akitada¹  Kisiel, Walter¹  Fujikawa, Kazuo¹ 

[1] Department of Biochemistry, University of Washington, Seattle, WA 98195, USA

关键词: Fibrinolysis;    t-PA;    Tissue kallikrein;    Coagulation factor;   

DOI  :  10.1016/0014-5793(84)80779-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tissue kallikrein and factor Xa were found to activate tissue plasminogen activator (t-PA) at a rate comparable with that of plasmin. During the activation reaction, the single-chain molecule was converted into a two-chain form. A slight t-PA activating activity was also found in plasma kallikrein. Other activated coagulation factors, factor XIIa, factor XIa, factor IXa, factor VIIa, thrombin and activated protein C had no effect on t-PA activation. t-PA was also activated by a tissue kallikrein-like enzyme that was isolated from the culture medium of melanoma cells. These results indicate that tissue kallikrein and factor Xa may participate in the extrinsic pathway of human fibrinolysis.

【 授权许可】

Unknown