Expression and bioregulation of the kallikrein-related peptidases family in the human neutrophil:

Innate Immunity

Alejandro JLizama¹ 

关键词: Tissue kallikrein;    KLKs;    kallikrein-related peptidases;    kinin B1 receptor;    bradykinin;    neutrophils;   

DOI  :  10.1177/1753425914566083

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

The family of kallikrein-related peptidases (KLKs) has been identified in a variety of immunolabeled human tissue sections, but no previous study has experimentally confirmed their presence in the human neutrophil. We have investigated the expression and bioregulation of particular KLKs in the human neutrophil and, in addition, examined whether stimulation by a kinin B1 receptor (B1R) agonist or fMet-Leu-Phe (fMLP) induces their secretion. Western blot analysis of neutrophil homogenates indicated that the MM of the KLKs ranged from 27 to 50 kDa. RT-PCR showed that blood neutrophils expressed only KLK1, KLK4, KLK10, KLK13, KLK14 and KLK15 mRNAs, whereas the non-differentiated HL-60 cells expressed most of them, with exception of KLK3 and KLK7. Nevertheless, mRNAs for KLK2, KLK5, KLK6 and KLK9 that were previously undetectable appeared after challenging with a mixture of cytokines. Both kinin B1R agonist and fMLP induced secretion of KLK1, KLK6, KLK10, KLK13 and KLK14 into the culture medium in similar amou...

【 授权许可】

CC BY